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Griffithsin inhibits Japanese encephalitis virus infection in vitro and in vivo

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Griffithsin inhibits Japanese encephalitis virus infection in vitro and in vivo

Auteurs : Hassan Z. A. Ishag [République populaire de Chine, Soudan] ; Chen Li [République populaire de Chine] ; Li Huang [République populaire de Chine] ; Ming-Xia Sun [République populaire de Chine] ; Fengjuan Wang [République populaire de Chine] ; Bo Ni [République populaire de Chine] ; Thowaiba Malik [République populaire de Chine] ; Pu-Yan Chen [République populaire de Chine] ; Xiang Mao [République populaire de Chine]

Source :

Archives of Virology [ 0304-8608 ] ; 2013-02-01.

RBID : ISTEX:BF06EC0CA56C7F30704E0463CA45376E4D368B30

Abstract

Abstract: Griffithsin (GRFT) is a broad-spectrum antiviral protein that is effective against several glycosylated viruses. Here, we have evaluated the in vitro and in vivo antiviral activities of GRFT against Japanese encephalitis virus (JEV) infection. In vitro experiments showed that treatment of JEV with GRFT before inoculation of BHK-21 cells inhibited infection in a dose-dependent manner, with 99 % inhibition at 100 μg/ml and a 50 % inhibitory concentration (IC50) of 265 ng/ml (20 nM). Binding assays suggested that binding of GRFT to JEV virions inhibited JEV infection. In vivo experiment showed that GRFT (5 mg/kg) administered intraperitoneally before virus infection could completely prevent mortality in mice challenged intraperitoneally with a lethal dose of JEV. Our study also suggested that GRFT prevents JEV infection at the entry phase by targeting the virus. Collectively, our data demonstrate that GRFT is an antiviral agent with potential application in the development of therapeutics against JEV or other flavivirus infections.

Url:

https://api.istex.fr/ark:/67375/VQC-RGNZ34G7-D/fulltext.pdf

DOI: 10.1007/s00705-012-1489-2

Affiliations:

République populaire de Chine, Soudan

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: Griffithsin (GRFT) is a broad-spectrum antiviral protein that is effective against several glycosylated viruses. Here, we have evaluated the in vitro and in vivo antiviral activities of GRFT against Japanese encephalitis virus (JEV) infection. In vitro experiments showed that treatment of JEV with GRFT before inoculation of BHK-21 cells inhibited infection in a dose-dependent manner, with 99 % inhibition at 100 μg/ml and a 50 % inhibitory concentration (IC50) of 265 ng/ml (20 nM). Binding assays suggested that binding of GRFT to JEV virions inhibited JEV infection. In vivo experiment showed that GRFT (5 mg/kg) administered intraperitoneally before virus infection could completely prevent mortality in mice challenged intraperitoneally with a lethal dose of JEV. Our study also suggested that GRFT prevents JEV infection at the entry phase by targeting the virus. Collectively, our data demonstrate that GRFT is an antiviral agent with potential application in the development of therapeutics against JEV or other flavivirus infections.</div>
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Griffithsin inhibits Japanese encephalitis virus infection in vitro and in vivo

Griffithsin inhibits Japanese encephalitis virus infection in vitro and in vivo

Source :

Abstract

Links toward previous steps (curation, corpus...)

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Pour manipuler ce document sous Unix (Dilib)

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